With the rapid development of renewable energy and new-type electrical load, the uncertainty of both sides on the source and load increases. This brings more challenges to the power grid peaking regulation and frequency control. Therefore, it is important and urgent to bring adjustable load into the real-time dispatching area to improve the operational balance and regulation capacity of the power grid. First, the feasibility of taking the adjustable load as the control object of automatic generation control (AGC) is analyzed. Then, taking electric vehicles into power grid frequency control as the application scenario, the system architecture of adjustable load participating in multi-level power grid real-time regulation is proposed. Through the construction of a security access area, the application of multiple load regulation terminals and other key technologies, real-time perception and adjustable load second-level control by a multi-level dispatching center are introduced. This increases the response speed of load participating in the power grid regulation from the minute to second level. The effectiveness and superiority of the proposed technical route are verified through a demonstration project of source-grid-load-storage multi-level coordinated dispatching and control in the Southwest China Power Grid. Finally, further research directions and development suggestions are put forward for the large-scale adjustable load normally participating in the real-time dispatching and control of the power grid. This work is supported by the Science and Technology Project of State Grid Corporation of China (No. 5400- 202027212A-0-0-00).