To improve the accuracy of fault location based on a steady-state feature and its resistance to transition resistance in a non-effective earthed system, a fault location method based on amplitude continuous regulation of zero sequence current is proposed. The injected signal is used to flexibly adjust the neutral voltage. Based on the test section of zero sequence current characteristic and the transition resistance, and the size of the adjustment coefficient of relationship, the scope of the regulation is determined. The steady-state amplitude can be obtained by amplifying fault features to different degrees. By superimposing the feature enhancement and comparing with the set threshold value, the fault section is located and the high-resistance fault section is identified according to the variation trend of zero-sequence current characteristics. The simulation shows that this method can eliminate the impact of a false zero-sequence amplitude component caused by three-phase imbalance, and can accurately locate the fault section for different transition resistances, and has strong applicability. This work is supported by the National Natural Science Foundation of China (No. 51737002) and the Changsha Science and Technology Project (No. kh1901140).