The electric vehicle has flexible charging time and good regulation performance. As a controllable load, the electric vehicle can participate in the load regulation of the microgrid, thereby avoiding the removal of the load due to insufficient power generation during the operation of the micro-grid island. This paper focuses on the reliability analysis of microgrids that take into account the charging control strategy of electric vehicles. Firstly, the minimum peak load model of electric vehicles is established to evaluate the regulation potential of electric vehicles in the microgrid operation period. Secondly, based on the minimum peak load model, an electric vehicle-energy storage joint regulation strategy considering power supply reliability is proposed. Thirdly, based on the Monte Carlo simulation method, a microgrid reliability assessment method considering the electric vehicle-energy storage combined regulation strategy is proposed. Finally, a numerical simulation is carried out through the modified RBTS Bus6 F4 feeder system. Simulation results verify the effectiveness of the proposed model and strategy. The results of the example show that the proposed microgrid regulation strategy can effectively reduce the impact of large-scale electric vehicle access on the reliability of microgrid on the basis of meeting the charging demand of electric vehicles. This work is supported by Science and Technology Project of China Southern Power Grid (No. ZBKJXM20160003).