At present, intelligent substations have been widely used, and the renewal of some standards and technologies has put forward higher requirements for the construction of intelligent substations. Through the comparative analysis of the secondary safety control measures of the conventional substation, this paper puts forward the relay protection composition and safety maintenance principles of the intelligent substation and analyzes the inspection methods of the secondary safety measures from the inspection of the main transformer and the line protection. The secondary safety measures system of the intelligent substation is modularized and divided into a graphic display module and a safety measures module to establish a safety measures anti-misoperation rule base and a one-button remote control operation. Compared with the traditional secondary safety measures, the one-button secondary safety measures in intelligent substations are more accurate and quick to realize the secondary safety isolation and anti-misoperation verification. This work is supported by Science and Technology Project of State Grid Corporation of China (No. 390034734600010).