It is hardly to ensure the power supply for all loads in microgrid with insufficient capacity of micro sources and energy storages during isolated mode. A dispatch strategy of microgrid operation based on demand side response and energy evaluation of energy storages is proposed to improve power supply reliability of microgrid and take advantage of renewable energy as far as possible. In this strategy, focused on microgrid operating in isolated mode, firstly, power and surplus energy of energy storages are estimated according to the supper short-term power prediction of micro sources and loads, and secondly, different scheduling strategies for all kinds of loads are implemented on the basis of the estimated result and demand side information. Through the optimization of shiftable loads and the shedding of interruptible loads with different grades, the goal for continuous power supply of the important loads and the shortest power-off time of rest loads is achieved. The simulation example verifies the correctness and effectiveness of the strategy proposed in this paper. This work is supported by National Natural Science Foundation of China (No. 51507139 and No. 51677151).