In order to guarantee the reliability and security of the communication network of smart substation, it must carry out the safety isolation transmission of communication business network flow. This paper analyzes the features of smart substation communication business flow, expounds the present commonly used network isolation technology, emphatically analyzes the application and characteristics of business separation technology of smart substation communication network, and points out urgent demand of communication service flow for the safe isolation. The main characteristic index and the classification of the mainstream technology of all optical switching technology are studied, the power business safety isolation scheme based on all-optical wavelength and optical label mixed switching technology is explored, and the implementation of measures and the technical principle of the program are introduced in detail. Through the introduction of business segregation evaluation model, the feasibility of the scheme is proved by way of experiment.