A new coordinated operation expert system and algorithm of hybrid energy storage and dispatchable generation is proposed. The purpose is to maximize the operating economy and prolong the battery’s lifetime, compared with the conventional cooperation model of single energy storage and dispatchable unit. Considering the running characteristics of hybrid energy storage and diesel generator, the dispatching of microgrid can be divided into upper level decision and lower real-time scheduling, then the reasonable combination operation mode of dispatchable unit is calculated. Depending on the equivalent cost, the microgrid operation costs, environmental benefit and energy storage life of quantitative are converted to a single objective function. Then improved hierarchical heuristic method and employed adaptive genetic algorithm(AGA) are used to optimize the power resources. The example shows that the system can effectively improve the efficiency of battery, prolong battery’s life and enhance the microgrid operation economy.