Based on Matlab/Simulink, the software for intelligent parameter identification of steam turbine and its governing system is developed, in which polynomial fitting, least square method, the simplex method, genetic algorithm, simulated annealing algorithm are applied. The pretreatment for the original data, intelligent extraction of actuator parameters identification sample, and intelligent processing of boundary conditions of load test data are performed automatically with this software. Furthermore, this software uses several algorithms to identify parameters, and selects the final results according to the preset logic. The simulation and verification results show that the parameters identified by this software can reflect the characteristics of actual steam turbine and its governing system. Finally, the key factors which influence the accuracy of the parameter identification are also indicated.